pe cy7 conjugated mouse igg isotype control  (Yeasen Biotechnology)

Journal: Journal of Innate Immunity

Article Title: Activation, Deficiency, and Reduced IFN-γ Production of Mucosal-Associated Invariant T Cells in Patients with Inflammatory Bowel Disease

doi: 10.1159/000507931

Figure Lengend Snippet: Decreased circulating MAIT cell proportions in peripheral blood of IBD patients. Freshly isolated PBMCs from 30 HCs, 22 patients with CD, and 18 patients with UC were stained with APC-Cy7-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2, and PE-Cy5-conjugated anti-CD161 mAbs and then analyzed by flow cytometry. Percentages of MAIT cells were calculated within a αβ T-cell gate. a Representative MAIT cell percentages as determined by flow cytometry. b MAIT cell percentages among peripheral blood αβ T cells. c Absolute MAIT cell numbers (per microliter of blood). Symbols represent individual subjects. Horizontal lines are median values. MAIT, mucosal-associated invariant T; IBD, inflammatory bowel disease; PBMC, peripheral blood mononuclear cell; HC, healthy control; CD, Crohn's disease; UC, ulcerative colitis; APC, allophycocyanin; FITC, fluorescein isothiocyanate; TCR, T-cell receptor; PE, phycoerythrin. *p < 0.05, **p < 0.005 by the ANCOVA test.

Article Snippet: The following monoclonal antibodies (mAbs) and reagents were used in this study: allophycocyanin (APC)-Cy7-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, fluorescein isothiocyanate (FITC)-conjugated anti-TCR γδ, FITC-conjugated anti-CD3, FITC-conjugated anti-IFN-γ, PE-conjugated anti-IL-17, PE-Cy7-conjugated anti-TNF-α, PE-conjugated anti-CD69, PE-conjugated anti-lymphocyte-activation gene3 (LAG3), FITC-conjugated mouse IgG isotype, PE-conjugated mouse IgG isotype, and PE-Cy7-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA, USA); PE-conjugated anti-programmed cell death-1 (PD-1; eBioscience, San Diego, CA, USA) and APC-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA, USA).

Techniques: Isolation, Staining, Flow Cytometry, Control